System location: HBTM 10th floor 10006B
To schedule a training session or pilot on the system, please contact Lai Ding ( lding@bwh.harvard.edu)
Reservation is through the Partners Core Management System (PCMS). Users need to register through the PCMS website (see below), then request training.
https://researchcores.partners.org/nts/about
Available Techniques: Fluorescent, Brightfield, DIC, Phase Contrast, Histology Color Imaging, Live-Cell Imaging, Whole-Slide Scan
Objectives: 2.5x, 5x, 10x, 20x, 40x (dry), 40x (water), 40x, 63x, 100x (oil)
The Leica DMi8 is a versatile widefield microscope system suitable for fluorescence, brightfield, contrast method (phase contrast, DIC, polarized), histology color imaging and live-cell imaging (with OKO lab on-stage incubator). The system can be used to image multiple sample types including slides, petri dishes, multi-well plates, and small flasks. It is also capable of large tile stitching and can be operated as a whole slide scanning instrument.
Download Leica DMi8 user manual by clicking
Download Leica DMi8 info sheet by clicking
Leica DMi8 Turn On Procedure
Leica DMi8 LAS X Startup procedure
Lif2Tif Converter
Lif2Tif convert all individual images in a Leica .lif file into Tiff format. Download “Lif2Tif” by clicking link below (change file extension from .txt to .ijm before running)
PUBLICATION ACKNOWLEDGEMENT: If support from the NeuroTechnology Studio results in a research paper or other public presentation, please acknowledge this support by including the following statement in your publication(s):
“We thank the NeuroTechnology Studio at Brigham and Women’s Hospital for providing [as applicable] instrument access and consultation on data acquisition and data analysis. “
Image Gallery
